Search results for "Reverse northern blot"

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Sex-specific windows for high mRNA expression of DNA methyltransferases 1 and 3A and methyl-CpG-binding domain proteins 2 and 4 in human fetal gonads

2006

DNA methyltransferases (DNMTs) and 5-methyl-CpG-binding domain proteins (MBDs) are involved in the acquisition of parent-specific epigenetic modifications in human male and female germ cells. Reverse Northern blot analyses demonstrated sex-specific differences in mRNA expression for the maintenance DNMT1 and the de novo DNMT3A in developing testis and ovary. In fetal testis DNMT1 and DNMT3A expression peaked in mitotically arrested spermatogonia around 21 weeks gestation. In fetal ovary transcriptional upregulation of DNMT1 and DNMT3A occurred during a very brief period at 16 weeks gestation, when the oocytes proceeded through meiotic prophase. Fetal gonads showed several fold higher DNMT3A…

DNA (Cytosine-5-)-Methyltransferase 1MaleMethyltransferaseEmbryonic DevelopmentGestational AgeOvaryBiologyGene Expression Regulation EnzymologicChromatin remodelingDNA Methyltransferase 3AFetal DevelopmentPregnancyTestisGeneticsmedicineHumansDNA (Cytosine-5-)-MethyltransferasesRNA MessengerEpigeneticsRegulation of gene expressionFetusReverse Transcriptase Polymerase Chain Reactionurogenital systemOvaryGene Expression Regulation DevelopmentalCell BiologyReverse northern blotMolecular biologyMethyl-CpG-binding domainCell biologyDNA-Binding Proteinsmedicine.anatomical_structureembryonic structuresFemaleTranscription FactorsDevelopmental BiologyMolecular Reproduction and Development
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